Dependence of polymerase chain reaction product inactivation protocols on amplicon length and sequence composition

Assessing Microbial Community Diversity Using Amplicon Length Heterogeneity Polymerase Chain Reaction

Lu21 O It is thought that a microbial community is an assemblage of organisms, genes, and gene functions. Transient, acute signals such as excessive nutrient loads or disturbance and chronic signals such as seasonal temperature or rainfall impact the total environmental system. The goal of many microbial ecologists is to determine if a finely resolved study of microbial dynamics can be used as ...

‘The Polymerase Chain Reaction and Sequence-tagged Sites

The polymerase chain reaction (PCR) is an in vitro methocl for selectively amplifying, or synthesizing millions of copies of, a short region of a DNA molecule. The reaction is carried out enzymatically in a test tube and has been successfully applied to regions as small as 100 base pairs and as large as 6000 base pairs. In contrast, DNA cloning is a nonselective in vivo method for replicating D...

Detection of Babesia ovis Using Polymerase Chain Reaction.

SPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION

Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...

Development of New Modified Simple Polymerase Chain Reaction and Real-time Polymerase Chain Reaction for the Identification of Iranian Brucella abortus Strains

Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents. In this regard, the identification of Brucella can be a time-consuming and labor-intensive process posing a real risk of laboratory-acquired infection to the laboratory staff. This stud...